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In order to run my microbial community samples from my experiments through DGGE, I was required to use multiple gels.
Thus it is necessary to compare banding patterns across more than one gel. This means that I need some way of assessing whether the 4th band on gel #1 is the same OTU as the 4th band on gel #2.
The industry standard for this procedure seems to be the Gelcompar software. Unfortunately this software is greater than $10,000 and is proprietary (thus, not open science friendly).
Does there exist a free (ideally as in speech and beer) alternative to Gelcompar?
Try this one
Pavel, A. B. & Vasile, C. I. PyElph - a software tool for gel images analysis and phylogenetics. BMC Bioinformatics 13, 9+ (2012).
You can download here http://sourceforge.net/projects/pyelph/